mouse pca cell line myc cap Search Results


human pca cell lines  (ATCC)
99
ATCC human pca cell lines
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Human Pca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pca cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
human pca cell lines - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
human pca cell lines lncap  (ATCC)
93
ATCC human pca cell lines lncap
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Human Pca Cell Lines Lncap, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pca cell lines lncap/product/ATCC
Average 93 stars, based on 1 article reviews
human pca cell lines lncap - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
trampc1 cells  (ATCC)
96
ATCC trampc1 cells
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Trampc1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trampc1 cells/product/ATCC
Average 96 stars, based on 1 article reviews
trampc1 cells - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
human pca cell lines pc 3  (ATCC)
99
ATCC human pca cell lines pc 3
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Human Pca Cell Lines Pc 3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pca cell lines pc 3/product/ATCC
Average 99 stars, based on 1 article reviews
human pca cell lines pc 3 - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
mda pca 2b  (ATCC)
96
ATCC mda pca 2b
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Mda Pca 2b, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mda pca 2b/product/ATCC
Average 96 stars, based on 1 article reviews
mda pca 2b - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
mouse pca cell lines rm 1  (ATCC)
96
ATCC mouse pca cell lines rm 1
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Mouse Pca Cell Lines Rm 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse pca cell lines rm 1/product/ATCC
Average 96 stars, based on 1 article reviews
mouse pca cell lines rm 1 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
cell culture mouse tramp c2 tc 2 pca cells  (ATCC)
96
ATCC cell culture mouse tramp c2 tc 2 pca cells
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Cell Culture Mouse Tramp C2 Tc 2 Pca Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell culture mouse tramp c2 tc 2 pca cells/product/ATCC
Average 96 stars, based on 1 article reviews
cell culture mouse tramp c2 tc 2 pca cells - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
human c4 2b pca cells  (ATCC)
97
ATCC human c4 2b pca cells
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Human C4 2b Pca Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human c4 2b pca cells/product/ATCC
Average 97 stars, based on 1 article reviews
human c4 2b pca cells - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
tramp-c2 mouse pca cells  (Charles River Laboratories)
90
Charles River Laboratories tramp-c2 mouse pca cells
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Tramp C2 Mouse Pca Cells, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tramp-c2 mouse pca cells/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
tramp-c2 mouse pca cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse pca cell lines  (ATCC)
94
ATCC mouse pca cell lines
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
Mouse Pca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse pca cell lines/product/ATCC
Average 94 stars, based on 1 article reviews
mouse pca cell lines - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
22rv1 pca cell line  (ATCC)
99
ATCC 22rv1 pca cell line
RelB deprivation in mouse <t>PCa</t> RM-1 cells restores mouse T <t>cell</t> function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell <t>lines</t> were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells
22rv1 Pca Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/22rv1 pca cell line/product/ATCC
Average 99 stars, based on 1 article reviews
22rv1 pca cell line - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
pca cell lines pnt1a  (ATCC)
99
ATCC pca cell lines pnt1a
Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in <t>PNT1A,</t> LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .
Pca Cell Lines Pnt1a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pca cell lines pnt1a/product/ATCC
Average 99 stars, based on 1 article reviews
pca cell lines pnt1a - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier

Image Search Results


RelB deprivation in mouse PCa RM-1 cells restores mouse T cell function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell lines were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: RelB upregulates PD-L1 and exacerbates prostate cancer immune evasion

doi: 10.1186/s13046-022-02243-2

Figure Lengend Snippet: RelB deprivation in mouse PCa RM-1 cells restores mouse T cell function. a RelB was knocked out in RM-1 cells using the CRISPR/Cas9-based gene-editing system and further PD-L1 was enforcedly expressed in RelB-deprived cells to restore the immunocompromise. The cellular levels of RelA, RelB and PD-L1 proteins in these cell lines were measured by immunoblots. b Subsequently, the NF-κB binding activities in the established cell lines were quantified. **( p < 0.01) shows significance between the two groups as indicated. c T cells derived from mouse spleen tissues were activated and CD4 + and CD8 + T cells were quantified by flow cytometry. d RM-1 cells were treated with an anti-PD-L1 mAb prior to coculture with activated T cells, and the RM-1 cell survival was measured using a clonogenic assay. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) shows significance in RelB-KO and RelB-KO/PD-L1 cells compared to control cells. e Correspondingly, the apoptosis of RM-1 cells was further analysed using flow cytometry. The significance between the two groups as indicated on the tap. Within the same groups, *( p < 0.05), **( p < 0.01) show significance in RelB-KO cells vs. the control cells, and # ( p < 0.05) shows significance in RelB-KO/PD-L1 cells compared to control and RelB-KO cells

Article Snippet: Human PCa cell lines (PC-3 and DU-145) and mouse PCa cell lines (RM-1) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Function Assay, CRISPR, Western Blot, Binding Assay, Derivative Assay, Flow Cytometry, Clonogenic Assay, Control

RelB deprivation decreases mouse xenograft tumour lung metastasis. a RM-1 and its generated cell lines were intravenously injected into male C57BL/6 mice. Lung tissues with tumours in the control group and without tumours in the RelB-KO groups were excised and examined. b The expression levels of RelB and PD-L1 in the excised tissues were measured by immunoblots. c Additionally, the expression of RelB and PD-L1 was further quantified by IHC and the relative H-scores were plotted. d The percentages of CD4 + and CD8 + T cells derived from mouse blood samples were analysed by flow cytometry. *( p < 0.05), **( p < 0.01) show significance between the two groups as indicated. e Depiction of the suggested mechanism underlying RelB-mediated immune evasion of PCa cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: RelB upregulates PD-L1 and exacerbates prostate cancer immune evasion

doi: 10.1186/s13046-022-02243-2

Figure Lengend Snippet: RelB deprivation decreases mouse xenograft tumour lung metastasis. a RM-1 and its generated cell lines were intravenously injected into male C57BL/6 mice. Lung tissues with tumours in the control group and without tumours in the RelB-KO groups were excised and examined. b The expression levels of RelB and PD-L1 in the excised tissues were measured by immunoblots. c Additionally, the expression of RelB and PD-L1 was further quantified by IHC and the relative H-scores were plotted. d The percentages of CD4 + and CD8 + T cells derived from mouse blood samples were analysed by flow cytometry. *( p < 0.05), **( p < 0.01) show significance between the two groups as indicated. e Depiction of the suggested mechanism underlying RelB-mediated immune evasion of PCa cells

Article Snippet: Human PCa cell lines (PC-3 and DU-145) and mouse PCa cell lines (RM-1) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Generated, Injection, Control, Expressing, Western Blot, Derivative Assay, Flow Cytometry

Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in PNT1A, LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Elongation and ROS-Mediated Apoptosis in Prostate Cancer Cells under Therapy with Apalutamide and Complex I Inhibitor

doi: 10.3390/ijms25136939

Figure Lengend Snippet: Treatment-induced changes in fission and fusion proteins. ( A , B ) Antibodies targeting OPA1 ( A ) and p-DRP1 (Ser616) ( B ) were quantified through automated Western blot analysis to determine changes in fusion and fission protein expression in PNT1A, LNCaP, C4-2 and PC-3 cells upon treatment with DMSO, ARN, IACS and Combo. The quantification of protein levels normalized to GAPDH is presented as means ± SEM ( n = 3). * p < 0.05; ** p = 0.0034; *** p < 0.001; **** p < 0.0001. ( C ) Representative virtual immunoblots are shown for OPA1 and p-DRP1 (Ser616). Uncropped lanes are shown in .

Article Snippet: PCa cell lines PNT1A (prostate epithelial cells), LNCaP (androgen-dependent) and PC-3 (androgen-independent) were all purchased from ATCC.

Techniques: Western Blot, Expressing

Assessment of apoptosis in PCa cells. ( A ) Representative flow cytometry dot plots of cells stained with Annexin V/PI. PCa cell lines PNT1A, LNCaP, C4-2 and PC-3 were treated for 72 h with DMSO, ARN, IACS and Combo. The sum of Annexin V (+)/PI (−) and Annexin V (+)/PI (+) cells in the lower right and upper right corner was used to quantify apoptotic cells. ( B ) Bar plots represent three independent experiments of measurements of apoptotic cells for PNT1A, LNCaP, C4-2 and PC-3. Bar plots are shown as means ± SEM. * p = 0.0179; ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Elongation and ROS-Mediated Apoptosis in Prostate Cancer Cells under Therapy with Apalutamide and Complex I Inhibitor

doi: 10.3390/ijms25136939

Figure Lengend Snippet: Assessment of apoptosis in PCa cells. ( A ) Representative flow cytometry dot plots of cells stained with Annexin V/PI. PCa cell lines PNT1A, LNCaP, C4-2 and PC-3 were treated for 72 h with DMSO, ARN, IACS and Combo. The sum of Annexin V (+)/PI (−) and Annexin V (+)/PI (+) cells in the lower right and upper right corner was used to quantify apoptotic cells. ( B ) Bar plots represent three independent experiments of measurements of apoptotic cells for PNT1A, LNCaP, C4-2 and PC-3. Bar plots are shown as means ± SEM. * p = 0.0179; ** p < 0.01.

Article Snippet: PCa cell lines PNT1A (prostate epithelial cells), LNCaP (androgen-dependent) and PC-3 (androgen-independent) were all purchased from ATCC.

Techniques: Flow Cytometry, Staining